![]() Genus Mycobacterium and MTB specific PCR targeting the mycobacterial hsp65 gene was used to confirm the discrepant results of GeneXpert and culture. Any acid-fast bacterial growth that was not detected as MTB by the immunochromatographic method was considered as NTM. Isolates were identified as MTB by their rate of growth, and immunochromatographic test kit (SD MPT64TB Ag kit). All negative tubes were incubated for at least eight weeks and then discarded. 15 All inoculated tubes were incubated in the MGIT 960 instrument (Becton Dickson and Company, Andover, MA, USA) until they were detected as positive by the machine. 12 The concentrated and decontaminated sample was emulsified in 1 mL of sterile phosphate buffered saline (pH 6.8) and 500 μL of the concentrate was inoculated into MGIT 960 tubes. 11 Therefore the present study is undertaken to compare the performance of GeneXpert and smear microscopy with mycobacterial growth indicator (MGIT) culture to choose the best available test for the diagnosis of TB.Ĭlinical samples from the non-sterile site were treated with N-acetyl-L-cysteine-NaOH and sterile specimens were used directly for culture after concentration. 10 Accurate and timely diagnosis of TB will reduce the transmission of the disease and unnecessary antibiotic use. Hence a sample with low bacterial count results in a negative report. However, it has low sensitivity and needs a concentration of 10000 colony forming units/mL to be seen as positive under a microscope. 7 - 9Īcid fast bacilli (AFB) smear is the least expensive and widely used diagnostic tool for pulmonary tuberculosis. 6 Most of the studies for the optimization and validation of the GeneXpert test are in patients with HIV infection from African and other countries and a few from India. 5 WHO strongly recommends Xpert assay for the diagnosis of TB and multidrug-resistant (MDR) TB in patients with HIV infection (WHO 2013). tuberculosis (MTB) and rifampicin resistance using molecular beacons within two hours. Xpert MTB/RIF, a programmed, cartridge-based real-time PCR, detects M. 2 - 4 Delay in diagnosis increases morbidity, mortality, and spread of disease in the community. 1 Commonly used diagnostic tests like smear microscopy and conventional culture have reduced sensitivity and long turnaround time, respectively. According to WHO Tuberculosis Report (2017), 24% of the global TB burden is seen in India. In India, tuberculosis (TB) is a community health problem. ![]()
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